Test Results are confidential, within limits of Iowa law.

Frequently Asked Question Regarding Johne's Testing
What are the most common diagnostic tests available for Johne’s disease?
  • Direct organism-based tests which detect Johne’s bacteria
    1. Fecal Culture - grows Johne’s organism from manure sample
    2. PCR - detects genetic material from Johne’s organism if present in the manure sample
  • Indirect or antibody tests, including ELISA, detect an immune response from exposure to all types of Mycobacteria, including Johne’s bacteria
What if an animal shows up positive to an ELISA

A positive ELISA classifies a herd as infected, but the ELISA test positive animal is considered a suspect. It is recommended that the suspect animal be confirmed by fecal culture within 45 days unless Johne’s has already been   confirmed on the premises.

What if an animal shows up positive to a fecal test?

A positive fecal culture or fecal PCR, which are official Johne’s disease tests, designate the affected animal as infected

A positive fecal culture or PCR is to be reported to the Department of Agriculture and Land Stewardship. Johne’s disease is a reportable but not a quarantineable disease.

The infected animal is restricted to the premise.

The infected animal is identified with a C-punch in right ear  (by the District veterinarian)

The identified infected animal can moved for the purpose of  slaughter only and must be accompanied by an owner-shipper  statement delivered to consignee.

An official JD test positive animal’s status may be appealed within 30 days of report, but is subjected to additional and extensive testing.